Chelex 100 resin pdf files

Choose biotechnology grade chelex 100 resin, a weak cation exchange media, for chelation, dna extraction for pcr, and other cation exchange protocols. The protocols i have recommend the use of chelex 100 resin which i dont have. How do i prepare chelex 100 suspension for dna extraction. Fill the wells in the heating block with water so block is never more. Add 1 g chelex 100 resin to water milliq or nanopure and bring up to a final volume of 10 ml. Chelex 100 is available from biorad 18004biorad, biorad chelex 100 resin, biotechnology grade, 100 200 mesh, sodium form, catalog 1432832 for 100 g. Pdf extraction of acanthamoeba dna by use of chelex. Chelex 100 resin is a styrene divinylbenzene copolymer containing paired iminodiacetate ions, which act as chelating groups in binding polyvalent metal ions. Because this is the first step towards pcr and amplifying your template dna, you must maintain excellent sterile technique. Vacuum or sweep up material and place in a disposal container section 7 handling and storage handling. Sr82 purification procedure using chelex100 resin article in applied radiation and isotopes.

Safety data sheet chelex 100 page 2 of 3 section 8 protection information acgih osha pel chemical name twa stel twa stel no data available na na na na control parameters engineering measures. The carboxylic acid groups of chelex 100 resin classify it as a weak cation exchange resin, but it differs from other exchangers in this class by featuring uniquely high selectivities for. Dna extraction experiences using chelex 100 from insects. Dna extraction experiences using chelex 100 from insects especially coleoptea. Chelex 100 as a medium for simple extraction of dna for. Safety data sheet chelex 100 page 2 of 3 no special spill cleanup considerations.